Journal: Scientific Reports

Article Title: MK2 inhibitor reduces alkali burn-induced inflammation in rat cornea

doi: 10.1038/srep28145

Figure Lengend Snippet: (A) Representative Western blots images shows the selective inhibitory effects of MK2i on MK2 activation on day 7 after alkali burn; (B) Summary plot showing changes in the MK2 phosphorylation status of the 4 groups; (C) MK2i does not inhibit p38 phosphorylation status after 7 days; (D) Summary plot showing changes in the p38 phosphorylation status of the 4 groups. Applications of either 15 or 150 μM MK2i fully and selectively suppressed MK2 activation. (Data are presented as Mean ± SEM, n = 3. ** p < 0.01, *** P < 0.001).

Article Snippet: After 1 h blocking in 5% BSA, the blots were incubated with primary antibodies for: MK2(1:1000, Cell Signaling Technology, Danvers, MA, USA), Phospho-MK2(p-MK2, 1:1000, Cell Signaling Technology), p38(1:200, Santa Cruz Biotechnology Biotechnology, Dallas, TX, USA), Phospho-p38(p-p38, 1:1000, Cell Signaling Technology), PEDF(1:500, Abcam), VEGF(1:1000, Abcam) and HRP-conjugated anti-β-actin antibody (1:20,000, Sigma, Saint Louis, MO, USA) as a loading control.

Techniques: Western Blot, Activation Assay